Experimental study on effect of tripterygium glycosides on T-lymphocyte subsets in rats with ITP / 中华中医药杂志

ABSTRACT

Objective:To study effect of tripterygium glycosides(TG)on T-lymphocyte subsets in rats with idiopathic thrombocytopenic purpura(ITP),and further to discuss the possible mechanism of TG in treating ITP.Methods:ITP animal model was established by intraperitoneal injection of exogenous anti-platelet–serum(APS).Sixty model rats were randomly divided into five groupsnormal group,saline group,the high and low dosage of TG groups,prednisone group.Every rat was giving liquid medicine by intragastric administration one time per day for ten days 36 hours after the first intraperitoneal injection of exogenous APS.The changes of peripheral hemogram,the amount of megakaryocyte of bone marrow and T-lymphocyte subsets were observed.Results:In high dosage of TG group,the number of platelet count increased obviously,the number of megakaryocyte in bone marrow,CD8+and CD4+decreased,and CD4+/CD8+increased.Conclusion:High dosage of TG had obviously therapeutic effect on IPT rat,such as increasing platelet count obviously,reducing number of megakaryocyte in bone marrow obviously and ameliorating T-lymphocyte subsets.The mechanism may be related to regulating the cell immune function.