Protective effect of propofol on tert-butyl hydroperoxide-induced apoptosis in cultured rat cardiomyocytes / 中国药理学通报

ABSTRACT

Aim To investigate the protective effects of propofol against tert-butyl hydroperoxide(t-BHP)-induced oxidative injury in cultured rat cardiomyocytes and the possible mechanism.Methods Primary cultured neonatal rat cardiomyocytes was performed.Cultured cardiomyocytes were divided into five groups control group,t-BHP group,and propofol 1,10,30 ?mol?L-1 group.Cellular Superoxide dismutase(SOD) activity,glutathione(GSH) and malonaldehyde(MDA) content were measured by colorimetric assay.Mitochondrial activity was determined by methylthiazolyl tetrazolium(MTT) test.The mitochondrial membrane potential(??m) was analyzed by Rhodamine123 staining and flow cytometry.The apoptosis of cardiomyocytes was detected by flow cytometry(FCM).The expression of caspase-3 was determined by Western blot.Results Compared with the control group,the MDA content significantly increased in t-BHP-treated group(P