E_2 upregulates HGF mRNA expression in rat heart during myocardial ischemia-reperfusion process / 第三军医大学学报

ABSTRACT

Objective To investigate the effect of 17?-estradiol (E2) on the expression of hepatocyte growth factor (HGF) mRNA in the myocardial tissues in rats during myocardial ischemia-reperfusion (I/R) process, and explore the relationship between HGF mRNA expression and myocardial apoptosis. Methods Using the random number table, 40 male SD rats were divided into 2 groups (20 rats in each group) randomly, ischemia-reperfusion (control) group and E2 treatment group. Myocardial I/R models of rats were duplicated by ligating the left anterior descending coronary artery for 20 min then reperfusion for 30 min. The expression of HGF and the apoptosis of myocardiocytes were observed with RT-PCR, TUNEL and flow cytometry. Results At the points of cardiac ischemia for 20 min and reperfusion for 30 min, in the E2 treatment group, the expressions of HGF mRNA were significantly higher than corresponding points of the control group (P