Therapeutic effect of mycophenolate mofetil on autoimmune encephalomyelitis in Wistar rats / 第三军医大学学报

ABSTRACT

Objective To investigate the therapeutic effect of mycophenolate mofetil (MMF) on rats with experimental autoimmune encephalomyelitis (EAE).Methods The guinea pig spinal cord homogenate (GPSCH) and complete Freund's adjuvant were used as the antigen to induce the acute EAE model in Wistar rats which were verified according to clinical scoring.Totally 36 rats were randomly and equally divided into 3 groups,that is,normal control,EAE group and MMF treatment group.In 10 d after immunization,normal saline or MMF (30 mg?kg-1?d-1) were given intragastrically for 7 d to the corresponding groups.The rats were sacrificed on the 16th day and 30th day after immunization for pathological changes of the brain and spinal cord.Clinical manifestation was scored during the experiment.Results Compared with the EAE model group,MMF-treated rats had lower clinical scores and shorter disease process.Besides,the inflammatory infiltration,demyelination and axonal damage in the central nervous system were significantly improved in MMF treatment group.Conclusion MMF is an effective drug in treating EAE,and can relieve the clinical signs and ameliorate pathological lesions in the central nervous system of acute EAE rats.