Bacterial homology in sterile packages for war readiness under natural environment in inland region / 第三军医大学学报

ABSTRACT

Objective To investigate the origin of bacteria in sterile packages for war readiness under natural environment in inland region.Methods Genomic DNAs from the Bacillus cereus,Micrococcus luteus and Staphylococcus cohnii ureal in sterile packages for war readiness and from air bacteria strains in war readiness warehouse were extracted,and then used as templates to amplify the 16S rRNA and 23S rRNA ITS(internal transcribed spacer) sequences by PCR.The PCR products were extracted and sequenced,and the sequences homology analysis was performed with BLAST.Results Amplification products of DNA PCR from Bacillus cereus,Micrococcus luteus and Staphylococcus cohnii ureal were about 800,1 200 and 1 000 bp respectively;The 16S rRNA-23S rRNA ITS sequences of bacterial strains in sterile packages shared 98% to 99% homology with those from the air bacteria strains in war readiness warehouse.Conclusion The bacteria in sterile packages for war readiness come from the air in war readiness warehouse under natural environment in inland region.