Research of Naokang Ⅱ in improving diabetic rat's cognitive impairment / 中华中医药杂志

ABSTRACT

Objective:To research the improvement of Naokang Ⅱ in treating diabetic rat's cognitive impairment.Methods:Naokang Ⅱ was used to treat diabetes.The change of rat's ability of learning and memory function was tested by water maze.The percentage of neuron apoptosis was detected through flow cytometry.The expression of Caspase-3 and Bcl-2 proteins in hippocampal CA1 region was measured with immunohistochemistry.Results:Naokang Ⅱ could significantly reduce the swimming time to platform and the percentage of neuron apoptosis,and increase the expression of Bcl-2 and decrease the expression of Caspase-3 in hippocampal CA1 region of model rats.Conclusion:Naokang Ⅱ could improve diabetic rat's cognitive impairment by reducing hippocampus neuron apoptosis and increasing the expression of Bcl-2 and reducing the expression of Caspase-3 in the hippocampus.