Effect of medicated serum of Radix Morindae Officinalis on skeletogenous cell bionomics / 中华中医药杂志

ABSTRACT

Objective:To explore the essence of Radix Morindae Officinalis in strengthening bones through observing the effect of medicated serum of Radix Morindae Officinalis on cultured osteoblast in vitro. Methods:The medicated serum of Radix Morindae Officinalis was added to the osteoblasts which were separated from the cranium of 24-hours newborn SD rat,in order to determining the effect of TGF-?1 and the proliferation and secreting of alkaline phosphatase and osteocalcin of the osteoblasts. Results:The proliferation of the osteoblasts were signifi cantly stimulated by the alcohol and the n-butanol extract from the Radix Morindae Officinalis; and the n-butanol extract from the Radix Morindae Officinalis could promote differentiating OB excreting the ALP and OC;in addition,the ability of the osteoblasts expressing TGF-?1 was promoted by the n-butanol extract from the Radix Morindae Officinalis. Conclusion:The n-butanol extract from the Radix Morindae Officinalis can promote the ability of the osteoblast in expressing TGF-?1 mRNA and excreting the ALP,and further promote the growth of bone.