Role of parathyroid hormone-related peptide in transdifferentiation of NRK-52E cells induced by high glucose / 第三军医大学学报
Journal of Third Military Medical University
; (24)2003.
Article
em Zh
| WPRIM
| ID: wpr-567769
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WPRO
ABSTRACT
Objective To study the role of parathyroid hormone-related peptide ( PTHrP) in transdif-ferentiation of NRK-52E cells induced by high glucose. Methods Expression of PTHrP and its receptor 1 in NRK-52E cells incubated with 0 ( control group) ,5 ( normal glucose group) and 16. 7mmol/L glucose ( high glucose group) ,respectively,was detected by RT-PCR and Western blotting,respectively. Expression of TGF-?1,MMP2,type Ⅰ collagen and ?-SMA in NRK-52E cells of normal and high glucose groups was detected by Western blotting and ROS was detected by CM2H2DCFDA after 72 h. Results The expression levels of PTHrP,PTHrP receptor 1 ( PTH1R) ,and PTHrP mRNA were higher in high glucose group than in control and normal glucose groups ( P
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WPRIM
Idioma:
Zh
Revista:
Journal of Third Military Medical University
Ano de publicação:
2003
Tipo de documento:
Article