THE EFFECT OF MICROFILAMENT INHIBITOR, CYTOCHALASIN D, ON ECa109 ESOPHAGEAL CANCER EPITHELIAL CELL / 解剖学报

ABSTRACT

The effect of cytochalasin D on ECa109 esophageal sqamous cancer cells was studied. When (ECa109) cells were treated with 0.2～1?g/ml cytochalasin D, small epithelial cells with a few microvilli could be seen. After incubation with 0.5?g/ml of cytochalasin D for one or three days, irregular buds containing endoplasmic reticulum, mitochondria and Golgi complexes grew out from the surface of the epithelial cells. The target-shaped mitochondria, the "confronting cisterna" of the endoplasmic reticulum, ring-shaped endoplasmic reticulum and disaggregated Golgi complexes were appeared in the cytoplasm. After treatment with 0.2?g/ml of cytochalasin D for one day multinucleated epithelial cells appeared. There were 2～6 nuclei, with different in size and shape. Some nuclei were fully enclosed with neuclear envelops and the others only partically. Mitotic cells could be seen too. The effect of cytochalasin D on the structure and function of the epithelial cells and the different roles played by the actin and tubulin in keeping the normal function of the cytoskeleton system of the ECa109 cells were discussed.