EFFECT OF HYPERTHERMIA ON ESOPHACEAL CANCER CELLS(ECa109) IN VITRO--FINE STRUCTURE OF INTERPHASE CELLS / 解剖学报

ABSTRACT

ECa 109 cells were heated to 42.5℃ for 60 min.or 90 min.,and to 44℃ for 30 min.The number of microvilli,desmosomes and RER decreased,but tonofilments increased.The polyribosome disaggregated as monoribosome.The mitochondria dilated and mitochondrial granules were observed.The Golgi complex disaggregated.Peri- chromatin granules and more dense heterochromatin were visible in the nucleus.The nucleoli were smaller,and the granular components were lost.The changes of organelles in cytoplasm appeared earlier than those in the nucleus. Recovery of the ultrastructural changes were also observed.After 37℃ for one/day, vacuous cells and microvilli increased in number significantly.Desmosomes,tonofilments, mitochondria,Golgi complexes,RER and polyribosomes all showed a recovering ten- dency.But,at the same time,a lot of lysosomes could be seen.On the fourth day of recovery,the structure of nuclear envelop remained indistint.The nucleoli varied in shape,with disappearance of granular components.During recovery,the organelles in cytoplasm restored earlier,while nucleic changes recovered later. The mechanism of ultrastructure changes induced by hyperthemia were discussed.