HISTOCHEMICAL OBSERVATION OF FUSION CELL BETWEEN HUMAN CANCER CELL LINE OF ESOPHAGUS AND CHINESE HAMSTER OVARY CELL LINE / 解剖学报

ABSTRACT

The Eca-109 cell line (human cancer cell line of the esophagus) and CHO cell line (Chinese hamster ovary cell line) were used in the present experiment. Three groups of the fusion cells induced by PEG were obtained. These groups were (1) the cells formed by the fusion of Eca-109 and Eca-109. (2) the fusion of CHO and CHO. (3) the hybrids of Eca-109 and CHO. The third group was demonstrated by autoradiography after incorporated H~3-thymine. The results indicate that the number of megalonucleated cells increases more and more, while the number of multinucleated cells gradually decreases in 10 days after the treatment by PEG in all groups.The forms of fusion cells of Eca-109 and Eca-109 or CHO and CHO similar to those of parent cells. Heterogeneous binucleated cells have the same form as one of parent ceils or as transitional form between two parent cells. The multinucleated cells have various shapes.Histochemical observations have shown that reactions of lactate dehydrogenase (LDH), glutamate dehydrogenase (GDH), malate dehydrogenase (MDH) in the fused cells were stronger than mononucleated cells. The level of activity of nonspecific esterase (NSE) in heterokaryon was intermediate between two parent cells i.e. higher than CHO and lower than Eca-109.