A Preliminary Study on Cultivation Test of Protoplast Fusion Strain of Fulin (Poria cocos) / 中草药

ABSTRACT

Rcsult of field cultivation test by protoplastic fusion technique for biological cngln ering breeding of Poria cocos in Huoshan county, Anhui Province was reported for the first time. A fusion strain F1 was obtained from parents P1, P6578 by separation, fusion, marker of hypha protoplast and identification of recombinat. It was cultivated simultaneously with P1, P5778 for comparison. Results showed that the average cellal outputs were 1.73, 0.5 and 2.20kg respectively.As the output of strain F1 is intermediate between its parent P1 and P578, the result is considered to be unsatisfactory. The reason for such result was discussed.