DIRECTIONAL INDUCEMENT OF SCIATIC NERVE DERIVED DIFFUSIBLE FACTORS ON REGENERATING AXONS OF ADULT FROGS / 解剖学报
Acta Anatomica Sinica
;
(6)1955.
Artigo
em Chinês
| WPRIM
| ID: wpr-569919
ABSTRACT
Objective To study the effects of directional inducement of factors produced by injured sciatic nerve on the regenerating axons of adult frog in vivo. Methods Conditioned medium with fraction of 30kD to 100kD was obtained from sciatic nerve of adult frog and pumped out and formed a gradient of concentration toward the end of central nerve stump of cutaneous pectoris nerve with different distances and angles by miniosmotic pumps.After 28\|35 days,the chest tissue was dissected and stained with immunocytochemistry to show the regenerated motor\|axons and the directional value is analyzed. Results The regenerated motor axons grew obviously towards the tip of the cannula with high concentration of CM.The directional value is 0^84 whereas the value is 0^04 with random direction.The average directional value between the two groups is significant and is not influenced by the distances and angles of the cannula to the nerve stump. Conclusion Damaged peripheral nerve can produce diffusible active factors with molecular weight between 30kD to 100kD,which induce the directional outgrowth of regenerating motor axons from sectioned nerve stump of adult frogs in vivo. [
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Acta Anatomica Sinica
Ano de publicação:
1955
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS