CHARACTERIZATION OF NEURAL STEM CELLS ISOLATED FROMHUMAN FETAL CEREBRAL CORTEX IN VITRO / 解剖学报
Acta Anatomica Sinica
;
(6)1955.
Artigo
em Chinês
| WPRIM
| ID: wpr-570490
ABSTRACT
Objective To establish human neural stem cells(HNSCs) model for further basic research and clinical application. Methods Cells from human fetal cerebral cortex were collected and cultured with serum free midium and then identified for nestin immunocytochemical staning;The cells were induced to differentiate by 5% fetal bovin serum and identified by neurofilament-200(NF-200) and glial fibrillary acidic protein(GFAP) immunocytochemical staning. Results The harvested cells appeared as clusters in suspension and both NF-200 and GFAP positive cells were observed after induction.After 12 generations of culture,these cells retained the main characteristics of NSCs.Conclusions The HNSCs were harvested from human fetal cerebral cortex and this HNSCs model can be used for futher basic research and clinical applications.;
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
Acta Anatomica Sinica
Ano de publicação:
1955
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS