The influence of protein-tyrosine phosphatase inhibitor on the vacuolating activity of VacA / 中华消化杂志

ABSTRACT

Objective It has been reported that VacA receptor of H pylori on gastric cell membrane is belong to the receptors of protein tyrosine phosphatase(RPTP) family, which are associated with signal transduction of cells. However the mechanism of vacuolation is still unknown. There may be a new way to reduce the toxicity of H.pylori by interfering the signal transduction. Methods The concentrates of supernatants from H.pylori liquid culture was used as rough VacA. Protein tyrosine phosphatase inhibitor (PTPI) and protein tyrosine kinase inhibit or(PTKI) which were diluted and combined with rough VacA were incubated with SGC 7901 cells for 24 hours. Results PTPI could inhibit the vacuolating activity with a minimal concentration of 2.7 ?mol/L( A 550 =0.46?0.06 vs 0.59?0.04, P