Isolation,purification and identification of polysaccharide of Cistanche deserticola / 中草药
Chinese Traditional and Herbal Drugs
; (24)1994.
Article
em Zh
| WPRIM
| ID: wpr-570798
Biblioteca responsável:
WPRO
ABSTRACT
Object The polysaccharide (CDPS) was isolated, purified and identified from Cistanche deserticola Y. C. Ma, a Chinese materia medica. Methods The polysaccharide was extracted with hot water and precipitated by alcohol. Protein in the precipitates was removed by Sevag method. The products were further purified with column chromatography on DEAE-Sephadex A-50 and Sephacryl S-200. The CDPS was idendified by IR spectrum and UV (200-400 nm) scanning spectrum. Results IR spectrum indicates that there are typical characteristic absorption peaks of polysaccharides. UV scanning spectrum shows that there are no absorption peaks of protein and nucleic acid at point 280 nm and 260 nm. Conclusion The CDPS was identified as homogeneous one.
Texto completo:
1
Índice:
WPRIM
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
Zh
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
1994
Tipo de documento:
Article