Study on fingerprint spectrum of Coptis chinensis by HPCE / 中草药

ABSTRACT

Object To establish the fingerprint spectrum of Coptis chinensis Franch. by HPCE. Methods The correlation relativity of retention time and peak area of each chromatographic peak in different batches of medicinal materials were explored respectively, and beberine was chosen as the reference to calculate the relative retention time and the ratio of peak area for each chromatographic peak. Results The correlation coefficients of relative retention time and the ratio of each chromatographic peak were all higher than 0.999 0 in different batches of medicinal materials. Conclusion The established fingerprint spectrum is stable and credible, and could be used as characteristic fingerprint spectrum for C. chinensis.