Seropharmacological Effect of Kangxian Ruangan Granule on Collagen-degradation Related Gene Expression in Hepatic Astrocytes / 中药新药与临床药理

ABSTRACT

Objective To study the effect of Kangxian Ruangan Granule(KRG)on the collagen degradation in hepatic astrocytes(HA)at the cellular and molecular level.Methods The medicated sera of KRG was used to i ncubate the sub-cultured HA and sodium chloride and c olchicines were taken as controls.T he content of typeⅠcollagen in the cultured su-pernatant was measured with ELISA an d then was corrected by assaying the t otal protein in cell layer.The mRNA e xpres-sions of matrix metalloproteinase -1(MMP1)and tissue inhibitor of metalloproteinase -1(TIMP -1)were analyzed by semiquantitative RT -PCR.Results The content of typeⅠcollagen in the KRG -cultured supern atant was lower than that in the other two groups(P