Study of the apoptosis of vascular endothelial cell and smooth muscle cell of rabbits induced hy 510.6 nm laser irradiation / 中华物理医学与康复杂志

ABSTRACT

Objective To observe the effects of 510. 6nm laser irradiation on the apoptosis rate of vascularendothelial cell and smooth muscle cell in rabbit. Methods After the abdominal aorta of rabbits were irradiated bydifferent dosages of copper vapor laser (100mW/cm~2?500s, 100mW/cm~2?1 000s, 150mW/cm~2?500s, 200mW/cm~2?500s, 200mW/cm~2?1 000s, 100mW/cm~2?500s), the apoptosis rate of vascular endothelial cells andsmooth muscle cells was detected by use of TUNEL staining. Results The apoptosis rate of vascular smooth musclecell in each laser group was 21. 2%, 24. 5%, 30. 8%, 37. 3%, and 34. 5% respectively, while that was 3. 4% inthe control group and there was significant difference between control group and each laser group (P 0. 05, respectively). Conclusion The irradiation of copper vapor laser would induce ahigh rate of apoptosis of vascular smooth muscle cell, while had no significant effect on endothelial cell at the same sit-uation.