Determination of tetrahydropalmatine in Juyanbaokang Granules by SPE-HPLC / 中成药
Chinese Traditional Patent Medicine
; (12)1992.
Article
em Zh
| WPRIM
| ID: wpr-571340
Biblioteca responsável:
WPRO
ABSTRACT
Objective: To determine tetrahydropalmatine in Juyanbaokang Granules (Rhizoma Corydalis, Radix Aconcti Lateralis Preparata, Radix Rauwolfiae, etc.) by SPE-HPLC. Methods : Samples were purified by C 18 SPE column. HPLC column was Dikma Diamonsil C 18 (250mm?4.6mm,5?m), mobile phase was 0.05M potassium dihydrogen phosphate-0.005M sodium heptanesulfonate-acetonitrile (1.2∶1.2∶1), flow rate was 1.2 mL?min -1 and detection wavelength was 230nm. Results : The calibration curve was linear in the range of 0.067 ~0.402?g for tetrahydropalmatine ( r =0.9997). The average recovery was 98.66%. Conclusion : The method with good separation is convenient, rapid, accurate and reliable.
Texto completo:
1
Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Traditional Patent Medicine
Ano de publicação:
1992
Tipo de documento:
Article