THE STUDY ON TRANSPLANTATION OF THREE-DIMENTIONAL CULTURED ASMC-COLLAGEN GEL TO SUBCUTANEOUS / 解剖学报

ABSTRACT

Objective To investigate if aortic smooth muscle cells (ASMCs) cultured three-dimentionally with collagen gel can still produce elastic fibers and the acceptance of hosts after transplantated in subcutaneous of rats. Methods AMSC-collagen gels that had been cultured for 2 weeks by three-dimentionally imitating the dermis substitute were transplanted to rats subscutaneous. After 4,7,10,14 and 28 days, the transplantations were examined via HE staining, Gomori aldehyde fuchsin staining, ?-actin immunohistochemistry and elastin immunohistochemistry. Results The amount of elastic fibers increased significantly in the week, whereas on the 28th day, no elastic fibers were detected.There was no remarkable leucocyte infiltration around the ASMC-collagen gel during 28 days.Conclusion Further studies are needed to explore the ASMC-collagen gels as a kind of elastic dermis substitute.