Detection of Chlamydia trachomatis DNA by gap ligase chain reaction (G-LCR) / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)1987.
Artigo
em Chinês
| WPRIM
| ID: wpr-571883
ABSTRACT
Objective:
To develop a new nucleic amplication method for detection of Chlamydia trachomatis DNA by gap ligase chain reaction(G-LCR).Methods:
A G-LCR DNA amplification assay that targeted the outer major membrane protein gene(omp1)of CT was established to detect CT infection.The sensitivity and specificity of a newly developed G-LCR test was examined by the use of highly purified elementary bodies (EBs).DNA fragments of different species and from other bacteria1 were detected with G-LCR and routine polymerase chain reaction (PCR).Results:
Using G-LCR,DNA fragments of 54bp were amplified from five different species.The sensitivity could be improved to detect out 2 chlamydial elementary bodies.G-LCR detected ten-fold EBs than PCR.No signal was observed when C.pneumoniae and other bacteria were used as templates.Conclusion:
G-LCR is sensitive,rapid and specific for detection of Chlamydia trachomatis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Journal of Chongqing Medical University
Ano de publicação:
1987
Tipo de documento:
Artigo
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