Effects of low energy intravenous He-Ne laser irradiation of blood on lymphocytes in patients with cerebral infarction / 中华物理医学与康复杂志

ABSTRACT

Objective To explore the effects of low energy intravenous He-Ne laser irradiation of blood (ILIB) on cell proliferation, apoptosis and genotoxicity of lymphocytes in patients with cerebral infarction (CI). Methods The lymphocytes in peripheral blood in patients with CI were sampled before and after treatment and cultured, and then, some parameters such as the mitosis index (MI), cell cyclical kinetics index (CCKI), sister chromatid exchanges frequencies (SCEF) and apoptosis were analyzed in order to explore the effects of ILIB on lymphocytes. Results The proliferative activities of lymphocytes were significantly increased by ILIB (P0.05). After ILIB, the rate of apoptosis in patients with CI was significantly increased when compared with that before treatment and that of the control group, as revealed by flow cytometry (FCM) (P