Nuclear Factor-kappa B Activation and Chemokine Genes Expression in HT-29 Intestinal Epithelial Cells in Response to Clostridium difficile Toxin A Stimulation
Journal of Bacteriology and Virology
; : 217-226, 2005.
Article
em Ko
| WPRIM
| ID: wpr-57228
Biblioteca responsável:
WPRO
ABSTRACT
Intestinal epithelial cells are known to up-regulate the expression of several chemokines in response to bacterial toxins. Since there has been little understanding on the cellular mechanisms of C. difficile toxin A-induced mucosal inflammation, we investigated whether nuclear factor-kappa B (NF-kappaB) could regulate chemokine gene expression in HT-29 intestinal epithelial cells stimulated with C. difficile toxin A. C. difficile toxin A rapidly increased signals of NF-kappaB composed with p65 and p50 subunits in HT-29 cells, whereas it decreased the signals of IkappaBalpha. Blocking the NF-kB activation by transfection with dominant negative I kappa B alpha-containing retrovirus attenuated the upregulated expression of IL-8, GRO-alpha, and MCP-1 induced by C. difficile toxin A. These results suggest that NF-kappaB is a major regulator of chemokine gene expression in C. difficile toxin A-stimulated intestinal epithelial cells.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Retroviridae
/
Toxinas Bacterianas
/
Transfecção
/
Expressão Gênica
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Clostridioides difficile
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Interleucina-8
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NF-kappa B
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Clostridium
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Células HT29
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Quimiocinas
Limite:
Humans
Idioma:
Ko
Revista:
Journal of Bacteriology and Virology
Ano de publicação:
2005
Tipo de documento:
Article