Determination of Allantoin in Rhizoma Dioscoreae from Different Producing Areas by Reverse- phase HPLC / 中药新药与临床药理
Traditional Chinese Drug Research & Clinical Pharmacology
;
(6)1993.
Artigo
em Chinês
| WPRIM
| ID: wpr-572293
ABSTRACT
Objective To establish a HPLC method for the determination of allantoin in Rhizoma Dioscoreae from different producing areas. Methods The RP- phase HPLC is applied. Separation and determination of allantoin was achieved by using an Allttima ODS C18 column(250 mm? 4.6 mm, 5? m) and a mobile phase of methanol- water (1 ∶ 39, V/V). The flow- rate was 0.8 mL/min, temperature at 30 ℃ and detection wavelength at 224 nm. Results A good linearity of allantoin was in the range of 0.24 ? g~ 2.40 ? g, r=0.9992; the average recovery rate was 103.1 % , RSD =2.0 % (n=6). Conclusion Rhizoma Dioscoreae from Henan Province is of the best quality and Rhizoma Dioscoreae from other provinces are only qualified for food.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Traditional Chinese Drug Research & Clinical Pharmacology
Ano de publicação:
1993
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS