The protective effects of estradiol on ischemic rat myocardium during the early stage of reperfusion / 中华胸心血管外科杂志

ABSTRACT

Ovbective To study the protective effects of 17?-estradiol on isolated ischemic rat hearts in a modified Langendorff model. Methods Thirty rats performed bilateral ovariectomies were divided equally into three groups at random. A three-phase protocol was performed (1) 15-minutes preperfusion,(2) 30-minutes global ischemia,(3) 30-minutes reperfusion. Treatment with 0.1% DMSO (group D) and 5 ?mol/L 17?-estradiol+0.1% DMSO (group E) dissolved in K-H buffer was perfused from sixth minutes of reperfusion. Only K-H was perfused in the ischemia-reperfusion group (group I-R). The left ventricular pressure (LVP) and it's first derivative (?dp/dt max ) and coronary flow (CF) were recorded at the 15th minute of preperfusion and the 30th minute of reperfusion. Creatin phosphokinase (CPK) and lactic dehydrogenase (LDH) the coronary effluent,the content of malonaldehyde (MDA) and superoxide dismutase (SOD) activity of myocardium were measured at the 30th minute of reperfusion. Results After 30-minute reperfusion,restoration of heart function of group E treated with 17?-estradiol were better than that of group I-R and group D,with a higher coronary flow,and a lower CPK and LDH level (P