Determination of Anabasine in Fengshiding Tablets by RP-HPLC / 中药新药与临床药理

ABSTRACT

Objective To determine the content of anabasine in Fengshiding tablets.Methods RP-HPLC method was established.Diamonsil-C18 was used as chromatographic column,the mobile phase consisted of methanol and 0.1 %triethylamine(5 ∶95,adjust pH to 3.6 with H3PO4),the flow rate was 1.0 mL?min-1 and the detection wavelength at 259 nm.Results The average recovery for Anabasine was 99.0 %,RSD=2.8 %(n=6).Conclusion The method can be used to determine the content of anabasine in Fengshiding tablets.