Study of Kanglaite-induced apoptosis on human pancreatic cancer cells by cDNA microarray / 中华消化杂志

ABSTRACT

Objective To assess the expression patterns of apoptosis-related genes in human pancreatic cancer cells (Patu-8988)induced by Kanglaite (KLT), a novel anti-cancer botanical product. Methods The apoptosis of Patu-8988 cells was determined by flow cytometry with Annexin V-FITC and propidium iodide staining. A human apoptosis microarray containing 96 cDNA fragments was used to detect gene expressions, and followed by Western blot analysis to confirm changes in expression of selected gene products. Results KLT induced apoptosis of Patu-8988 cells in a time-dependent manner. cDNA microarray analysis identified 5 down-regulated genes and 12 up-regulated genes (more than three fold) in the first 24 h as a consequence of treatment. Western blot performed for P53, Bcl-2, Bax and Caspase-3 were consistent with the microarray results. The enhanced activity of Caspases-3 was evidently verified by the cleavage of the 89?103 form of poly ADP-ribose polymerase. Conclusion KLT could alter the expression profile of apoptosis- related genes in human pancreatic Patu-8988 cancer cells, which provides valuable insight into the tentative signaling pathways mediating the outcome of KLT-induced apoptosis.