Effects of early intervention on functional outcome and content of GAP- 43 in the brain of rats with hypoxic-ischemic brain damage / 中华物理医学与康复杂志

ABSTRACT

Objective To study the effects of early intervention on functional outcome and content of growth associated protein (GAP- 43) in the brain of rats with hypoxic-ischemic brain damage (HIBD). Methods The animal model of intra-uterus HIBD was established in 30 pregnant Sprague-Dawley rats, who were then divided randomly into 4 groups the intervention HIBD group (HIBD-IT),the non-intervention HIBD group (HIBD-NIT),the intervention control group(control-IT),the non-intervention control group(control-NIT). From the second day after the operation, the rats of the HIBD-IT and control-IT groups were kept in an enriched environment and administered with the neonatal handling including massage for 14 days. On the 28th day, jumping stand test was administered to all the rats, at the same time, the expression of GAP- 43 in brain of the rats in different groups were measured by immunohistochemical method. Results On the jumping stand test, the performance of control-IT and HIBD-IT groups were significantly better than that of the control-NIT and HIBD-NIT groups(P