Determination of terpenelactones in Yinxingye Soft Capsule by HPLC-ELSD / 中成药

ABSTRACT

AIM: To determine the content of terpenelactones in Yinxingye Soft Capsule(Folium Ginkgo). METHODS: HPLC with Evaporative Light Scattering Detector (ELSD) was established to determine the content of terpenelactones in Yinxingye Soft Capsule. The analysis was carried out on C 18 column. The mobile phase was 1-propanol-THF-water(1∶15∶84). The operating conditions of the ELSD were a nebulizer nitrogen flow-rate of 3.5L?min -1 and an evaporator tube temperature of 115℃. RESULTS: No interference was found in the terpenelactones peak position. The method was simple, with a good linear relationship. The relative standard deviation of reproducibility was 1.04% and the average recovery was 97.1%. CONCLUSION: The method can be used for quality control.