The clone of human LIF gene and construction of its eukaryotic expression vector / 重庆医科大学学报

ABSTRACT

Objective:To clone LIF gene for the construction of mammalian expression vector PCDNA3.1(+)-LIF.Methods:LIF gene was cloned from pregnant uterine decidua tissues by using RT-PCR method,the gene was ligated with T-vector,then LIF gene was excised from T-LIF plasmid and inserted into PCDNA3.1(+) to construct eukaryotic expression vector consisting of LIF gene.Results:LIF gene sequence and vector were verified correctly by enzyme digestion,sequence analysis.Conclusion:The successful clone of LIF gene and the construction of its mammalian expression vector has been obtained.The results have provided basis for the further studies on LIF.