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Preparation of immunoliposomes encapsulating alkaline phosphatase / 重庆医科大学学报
Journal of Chongqing Medical University ; (12)1987.
Artigo em Chinês | WPRIM | ID: wpr-573205
ABSTRACT

Objective:

To prepare immunoliposomes encapsulating alkaline phosphatase and to evaluate the methods.

Methods:

The liposomes containing alkaline phosphatase were prepared by reverse phase evaporation or film dispersion.Liposomes and rabbit anti-human IgG were conjugated by glutaraldhyde or deriving antibody.Not only did the liposomes encapsulate alkaline phosphatase but also they had immune activity.All methods were analyzed and compared.

Results:

Many liposomes prepared by reverse phase evaporation had perfect shape and high bioactivity of alkaline phosphatase.The glutaraldhyde method conjugating antibody was convenient,efficient and practical.

Conclusion:

Immunoliposomes with bioactivity of enzyme and antibody are prepared through reasonable methods.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Journal of Chongqing Medical University Ano de publicação: 1987 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Journal of Chongqing Medical University Ano de publicação: 1987 Tipo de documento: Artigo