IDENTIFICATION AND CHARACTERIZATION OF CD34~+ STEM CELLS OF HEMATOPOIETIC TUMOR CELL LINES / 解剖学报

ABSTRACT

Objective To identify and characterize the CD34~+ stem cells of hematopoietic tumor cell lines. Methods Taking CD34 molecule as a marker to identify if there are CD34~+ tumor cells in hematopoietic tumor cell lines(THP-1,MEL,K562,MOLT-4,RAJI,RPMI8226,SP2/0 and DCS) by fluorescence staining and FCM technology. We separated CD34~+ cells from K562 and MOLT-4 for further study.CD34~+ cells were cultured and observed their development.CD34~+ and CD34 +- cells were compared in their functional status(mRNA),cell cycles(G -0/G -1),their degree of differentiation(Id-1 expression) and their multi-drug resistance(gp expression). Results The percentages of CD34~+ cells were 6