Qualitative and quantitative analysis of aristolochic acids in Chinese materia medica and traditional Chinese patent medicines / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Artigo
em Chinês
| WPRIM
| ID: wpr-573946
ABSTRACT
AIM:
To establish identification and determination methods for aristolochic acids.METHODS:
The extracts of the sample were developed on silica gel G F254 plate, using supernatant layer of the mixture of toluene-ethyl acetate-water-formic acid (20 ∶10 ∶1 ∶1) as the mobile phase. Chromatogram systemgradient elution with mobile phase consisting of (A) 1% acetic acid in water and (B) methanol was used. The initial condition was at 40% of B and gradient up to 100% B in 15 minutes before returning to the initial conditions. Detection was at 310 nm. The column temperature was set at 40℃ and flow-rate was set at 1.0 mL/min.RESULTS:
TLCthe Rf value of aristolochic acid Ⅰ was 0.50 and aristolochic acid Ⅱ was 0.53. The results of assay were shown as follow The average recovery of aristolochic acid Ⅰ was 103.3% (RSD=0.98%). The average recovery of aristolochic acid Ⅱ was 95.97% (RSD=1.2%).CONCLUSION:
It reveals that the methods we described are accurate, rapid and reproducible.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Pesquisa qualitativa
Idioma:
Chinês
Revista:
Chinese Traditional Patent Medicine
Ano de publicação:
1992
Tipo de documento:
Artigo
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