Preparation of ~(99m) Tc-MAG_3-ASON and distribution in breast carcinoma-bearing nude mouse model / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)1986.
Artigo
em Chinês
| WPRIM
| ID: wpr-573976
ABSTRACT
Objective:
To discuss the preparation of ~(99m) Tc-labelled antisense oligonucleotide (ASON) and its distribution in breast carcinoma-bearing nude mouse model.Methods:
N-hydroxysuccinimide ester of mercaptoacetylglycylglycylglycine (NHS-MAG_3) was synthesized by one step.A 15-mer single-stranded phosphorothioate ASON complementary to c-erbB2 oncogene mRNA was coupled with NHS-MAG_3 and labelled with ~(99m) Tc subsequently.~(99m)Tc-MAG_3-ASON was purified on a gel filtration column of Sephadex G25,then its stability was evaluated,and finally the distribution was detected in breast carcinoma-bearing nude mice model.Results:
The average labeling efficiency of 99mTc-MAG_3-ASON was 70.6%,and the radiochemical purity was 94.3% after being labelled at room temperature for 4h. When incubated with fresh human serum, the radiochemical purity of ~(99m) Tc-MAG_3-ASON was 93.8%. The serum protein binding of ~(99m) Tc-MAG_3-ASON was 11.8%.The highest uptake of breast carcinoma was 6.09%ID/g at 2hs.Conclusion:
~(99m)Tc-MAG_3-ASON has desirable stability,higher uptake in breast carcinoma and may provide a new sensitive tool for diagnostic imaging and therapy for breast carcinoma in the future.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Chongqing Medical University
Ano de publicação:
1986
Tipo de documento:
Artigo
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