Analyze the associativity between Helicobacter pylori infection and cholecystolithiasis / 重庆医科大学学报

ABSTRACT

Objective:To investigate the relationship of H pylori with cholecystolithiasis,to collect the evidence of Hp existing in biliary tract,and to study the detective method of Hp DNA in billiary tract.Methods:Hp which existing in bile,mucosa and gallstone of 120 cholecystolithiasis patients were cultured according to the literature.Then they were amplified by the primer of Ure A and cagA.Amplified products were analyzed.Results:All of the 120 samples were negative.No Hp grew in the medium.No Hp DNA and Hp Cag A antibody were found with PCR method.Conclusion:(1) It was restricted by many factors that Hp DNA in bile and gallstone wereexamined with PCR.(2) Hp maybe entered biliary tract regressively through Oddi's sphincter andhad no relationship with cholecystolithiasis.