Gastric-mucosal-barrier Protective Mechanism of Hewei Jiangni Decoction for the Treatment of Stress Ulcer / 广州中医药大学学报

ABSTRACT

[Objective] To observe the effect of Hewei Jiangni Decoction (HJD) for the treatment of stress ulcer and to explore its possible mechanism. [Methods] Sixty SD rats were randomized into 6 groups normal, model, ranitidine (0.05 g?kg-1?d-1), and HJD groups (1.25, 2,50 and5.00g?kg-1?d-1 respectively). Except the normal group, the rats in other groups were treated with water immersion method to induce stress ulcer. Then the drugs according to the experimental design were administered to ranitidine and HJD groups for 5 days successively , and the model and normal groups were given saline. Gastric mucosal blood flow was detected by laser Doppler flowmeter, and inducible nitric oxide synthetase (iNOS) mRNA expression in gastric mucosa was examined by reverse transcriptase polymerase chain reaction (RT-PCR) . [Results] HJD decreased ulcer index, increased gastric mucosal blood flow (GMBF), and reduced iNOS mRNA expression in the gastric mucosa, the difference being significant ( P