Identification on Radix Polygoni Multiflori and Radix Cynanchi Auriculati by HPLC fingerprint / 中草药

ABSTRACT

Objective To establish HPLC fingerprint for the identification of Radix Polygoni Multiflori and Radix Cynanchi Auriculati. Methods Chromatographic fingerprint of Radix Polygoni Multiflori, Radix Cynanchi Auriculati, Radix Polygoni Multiflori Preparata, and "HESHOU WU FEN" was determined by RP-HPLC (DAD) and the gradient elution mode applied in chromatographic separation, data were analysed by Fingerprint Similarity Evaluation Software to compare the similarity of samples. Results Radix Polygoni Multiflori from different samples were of high similarity, but Radix Polygoni Multiflori and Radix Cynanchi Auriculati showed evident difference in fingerprint, and there was difference in fingerprint through processing them. Conclusion HPLC fingerprint method is repeatable and feasible and can be used for the identification of Radix Polygoni Multiflori and Radix Cynanchi Auriculati.