Preparation of brucine liposome by a combination of ammonium sulfate transmembrane gradients method and extrusion through microfiltration membranes / 中成药

ABSTRACT

AIM: To preparate brucine liposome. METHODS: Brucine liposomes were preparated by a combination of ammonium sulfate transmembrane gradients method and extrusion through microfiltration membranes.The methods were evaluated by particle morphology,the size range,and encapsulation efficiency. RESULTS: The optimal preparating process parameters of brucine nanometer liposome were lecithin-to-cholesterin ratio of 100∶15,the volume of 0.1 mol/L of water solution of ammonium sulfate being 66.7 times as large as lecithin,the volume of brucine being 0.0167 times than lecithin,the temperature and the time of incubating being 40 ℃ and 20 min,respectively.The encapsulation efficiency of this method was over 90%. CONCLUSION: Preparation of brucine liposomes by a combination of ammonium sulfate transmembrane gradients method and extrusion through microfiltration membranes is feasible.