THE ROLE OF CALCIUM IN Nogo-A INHIBITING AXONAL OUTGROWTH / 解剖学报

ABSTRACT

Objective To explore the role of intracellular Ca~(2+) in inhibition of axonal outgrowth,which could learn more about the signal transduction mechanism of Nogo-A inhibiting axonal outgrowth. Methods Using laser confocal microscope,intracellular calcium level in cerebellar granule neurons after Nogo-A added in different times was measured and the effect of Nogo-A inhibiting axonal outgrowth when priming nimodipine was observed. Results The concentration of Ca~(2+) increased in 5 minutes after Nogo-A added,and reached to peak in 30 minutes,then decreased gradually,90 minutes later dropped to normal level.When priming with nimodipine,the axonal inhibition by Nogo-A was partly abrogated compared with control.Conclusion Ca~(2+) may participate in the signal transduction of Nogo-A inhibiting axonal outgrowth.