Reinforcement of anticancer effect of combined mitomycin and bombesin on human gastric cancer cell in vitro / 重庆医科大学学报

ABSTRACT

Objective: To study the effects of combined mitomycin(MMC) and bombesin(BOM) on apoptosis of human gastric cancer cell in vitro.Methods: Four groups were formed control group,MMC group,BOM group and MMC+BOM group.The effects of different concentrations of MMC and BOM,on the rate of cell existence,and cell apoptosis in human gastric cancer line SGC 7901 were studied in four groups by using cell culture,MTT assay and flow cytometry.Results: Within the scope of certain density,the multiplication effect of BOM,and the inhibitory action of MMC on stomach cancer cell line SGC 7901 were in a time and dose dependent manner.The rates of cell existence in four groups were respectively 90.00% in BOM group?82.96% in control group?65.07% in MMC group and 60.07% in MMC+BOM group,lowered one by one in order.There was significant difference in statistics about the rate of apoptosis between MMC+BOM group(29.26%) and control group(4.72%) or BOM group(5.13%)(P