Effect of drugs on spermatogenesis following testicular ischemia-reperfusion in rats / 中华急诊医学杂志

ABSTRACT

Objective To investigate effects of drugs on spermatogenesis following testicular ischemia-reperfusion in rats. Methods Healthy male Sprague-Dawley rats ( n = 32) were randomly divided into four groups, 8 rats in each group. Animals underwent unilateral testicular torsion followed by detorsion after 2 hours. Isotonic saline, pentoxifylline and verapamil were infused into tail vein 15 minutes before detorsion in torsion group, pentoxifylline group and verapamil group. At 24 hours after operation, testicular function was determined measuring germ cell apoptosis using the flow cytometry ( FCM) , and the levels of myeloperoxidase ( MPO) , superoxide dismutase (SOD) and malondialdehyde (MDA) by spectrophotometry. Results Compared with torsion group, the number of apoptotic germ cell and the contents of MDA and MPO were markedly decreased in pentoxifylline group and verapamil group, but the number of haploid and the level of SOD were significantly increased (P