Determination of Chlorogenic Acid from Sambucus chinese in Various Collecting Periods / 中药新药与临床药理

ABSTRACT

Objective To supply evidence for determining the optimal collecting period for Sambucus chinese.Methods A RP-HPLC method was established to determine chlorogenic acid contents from Sambucus chinese in various collecting periods.The chromatographic conditions were as follows Hypersil-ODS2(250 mm? 4.6 mm,5 ? m)column,acetonitrile-1 % formic acid aqueous solution(9 ∶ 91) as mobile phase with a flow rate of 1.0 mL? min-1,column temperature at 35 ℃ and the detection wavelength at 326 nm.Results Content of chlorogenic acid in the samples collected in May(pre-blossom period)was the highest and the content in leaf was higher than in the stem and root.Conclusion It is suggested that Sambucus chinese should be gathered before blooming when the leaves are in luxuriance.