Orthogonal design for optimizing the semi-bionic extraction of Simiao Yongan Decoction / 中成药

ABSTRACT

AIM: To investigate the method for using semi-bionic extraction (SBE) on Simiao Yongan Decoction as an example. METHODS: Orthogonal design was used to optimize the extraction conditions of SBE with the evaluation markers such as chlorogenic acid, ferulic acid, glycyrrhizic acid, total polysaccharide, ethanol soluble extractives and dried extract. RESULTS: The optimized SBE conditions were as follows The medical material was powdered through a sieve with 4 meshes) and extracted with 20% ethanol for three times, the pH of the three extractions were in proper order 3.50, 7.50, 8.50 ; the solvent volume was 10,8,8 times, respectively, and the extraction time was 1.5,1.0,1.0 h, respectively. CONCLUSION: The optimum extraction conditions are stable and practicable, and surpass the original SBE extraction conditions.