Expression of GABAAR?1 subunit in cortical region and hippocampal formation of rats with radiation-induced diffuse cortical dysplasia / 重庆医科大学学报

ABSTRACT

Objective:To correlate diffuse cortical dysplasia(DCD) epileptogenicity and the expression of GABAAR?1 in adult rat with DCD by ?-ray irradiated injury.Methods:To establish ?-ray-induced model of DCD in rat,and newborn Wistar rats were allowed to survive for 14~16 weeks before HE,Nissl Stain,Timm's histochemical method and SABC immunohistochemistry.We observed the structure of cortical regions and hippocampal formation with naked eyes and under a light microscope,and assessed the hippocampal mossy fiber sprouting after HE staining,Nissl staining and Timm's histochemical method.The comparisons between every two groups were performed with Nemenyi test.We observed the expression of GABAAR?1 subunit in cortical regions and hippocampal formation in the animal model and quantitatively analyzed using immunohistochemistry of GABAAR?1 subunit.Results:Normal rats and irradiated pregnant rats didn't have any seizure.F1 generation rats had seizure by chance.EEG showed no typical sharp waves,or spikes.In utero irradiation of fetal rats had served as an injury-based animal model of cortical dysplasia and neuronal heterotopia.Exposure of fetal rats to a single dose of ?-irradiation on embryonic day 17 resulted in microcephaly,diffuse cortical dysplasia,neuronal heterotopia,heterotopic neurons in the hippocampus,and hypoplasia of the corpus callosum.The mossy fiber sprouted in hippocampi CA3 area bilaterally,however there was no mossy fiber sprouting in controls or irradiated rats(P