Optimization of the Extraction Procedures of Compound Three-Flower-Tea by Orthogonal Design / 中国中医药信息杂志

ABSTRACT

Objective To optimize the extraction procedures of compound Three-Flower-Tea (CTFT) by orthogonal design. Methods After having established the methods of quantifying the chlorogenic acid by TLC-UV, the orthogonal design was used to select the best extraction procedures, using chlorogenic acid content and extract yield as the measuring index, through controlling four different factors, naming extraction temperature (A), extraction time (B), watervolume added (C) and extraction number of times (D), and three levels at each factor. Result The effective factors which influenced the extraction procedures were A, C and D. Conclusion The optimum extraction procedures for CTFT should be 15-fold water volume, three-time decocting with 20 minutes per time at temperature of 100 ℃.