Protective function of L-carnitine against valproate-associated hepatotoxicity in infant rats and its mechanism / 重庆医科大学学报

ABSTRACT

Objective:To explore the protective function of L-carnitine against valproate-associated hepatotoxicity in infant rats and its mechanism.Methods:Rat models with VPA liver lesion were established by oral administration of VPA and PB.L-carnitine was adopted as intervention measure:to observe its protection of experiment rat livers,especially the function of liver mitochondrion.Levels of plasma ammonia,L-carnitine and coagulation factors in sera as well as the changes of respiratory enzymes in hepatic mitochondria were measured by chemical colorimetry. Mitochondrial membrane potential(MMP),and VPA and PB blood drug levels in liver were determined by flow cytometer and HPLC,respectively.and morphological changes of hepatocytes were observed under microscope with Oil-Red-O staining.Results(1)In the groups affected by VPA or VPA added with PB,the activities of SDH and CCO significantly decreased compared with control group(P