Expression change of TGF-?1 and MMPs in rats with hyperoxia-induced lung injury / 重庆医科大学学报

ABSTRACT

95%),the dynamic expressions of TGF-?1,MMP-2 and MMP-9mRNA in lung for each hyperoxia group were measured by reverse transcript polymerse chain reaction(RT-PCR).The protein content in bronchoalveolar lavage fluid(BALF),permeability index,lung wet/dry weight ratio and pathological changes of lung tissues were all tested.Results:The level of TGF-?1mRNA in hyperoxia group increased from the 3rd day,and rose gradually until the 14th day.The expressions of MMP-2 and MMP-9 mRNA increased markedly from the 3rd day,rose to the peak at the 7th day,and decreased at the 14th day.The above changes accompanied with lung pathological manifestations,which included acute alveolitis during the earlier period and lung fibrosis during later period.Conclusions:The unbalance between TGF-?1 and MMPs may play an important role in the development of lung fibrosis caused by hyperoxia-induced lung injury.