Experimental study on ossification of deproteinized osteoarticular allograft with calcium phosphate cement containing recombinant human vascular endothelial growth factor and bone morphogenetic protein-2 in dogs / 重庆医科大学学报

ABSTRACT

Objective:To explore the ability of osteo-articular allograft of variant deproteinized bone combining calcium phosphate cement with recombinant human vascular endothelial growth factor and bone morphogenetic protein-2 in repairing osteo-articular defect.Methods:36 adult mixtus dogs were constructed as semi-articular defect models through resecting 30mm right femur inferior extremity near articular facet,and they were randomly divided into 3 groups.In group A,material CPC/rh VEGF/rh BMP-2/DBP was transplanted into the semi-articular defect.In group B,material rhVEGF/rhBMP-2/DBP was transplanted there.In group C,simple material DPB was transplanted there.At 4,8,12 and 16 weeks after operation,the effects were as- sessed by macro-morphology observation,X-ray examination,histologic examination as well as collagen type I immunohistochem- istry analysis transmission electron microscope examination and vitodynamic tests in order to observe bone reparation matter. Results:The new bone formation observed by vitodynamic tests,macro-morphology observation,X-ray examination,histologic ex- amination and transmission electron microscope examination in group A was significantly superior to that in group B and C.The results of collagen type I immunohistochemistry analysis and vitodynamic tests were significantly superior to that in group B and C(P0.05).Conclusion:The mate- rials CPC/rh VEGF/rh BMP-2 can promote the new bone formation,achieve bone healing and make them become autologous tissue at last.