The effect of mobilization of ginseng polysaccharide on murine hematopoietic stem/progenitor cell / 重庆医科大学学报

ABSTRACT

Objective:To study the mobilizable effect of ginseng polysaccharide(GPS)on murine hematopoietic stem/ progenitor cells and provide experimental evidences for mobilizable preparation of hematopoietic stem cell from plant polysaccharide.Methods:The changes of the numbers of WBC and MNC,the percentage of CD34+ cell and the yields of CFU-Mix in peripheral blood were examined by means of WBC and MNC counts,immunocytochemistry,flow cytometry and hematopoietic cell culture in vitro.Results:4 hours after injection of GPS into mice with a dosage of 2 mg/kg,the numbers of WBC and MNC reached the maximum,which were 3 times and 3.5 times that of the NS group respectively.The percentage of CD34+ cell and the yields of CFU-Mix in vitro in peripheral blood(0.0079?0.0011 and(25.0?9.1)cfu/2?105MNC respectively)after the mobilization by GPS were obviously higher than those of the NS group.The effect of mobilization by combination of GPS and G-CSF was much better than that mobilized by single administration of GPS.Conclusion:GPS alone or in combination with G-CSF could significantly mobilize murine hematopoietic stem/progenitor cells from bone marrow into peripheral blood.